Differential gene expression in aortae isolated from mice consuming normal water or that containing polystyrene beads

Microplastics (MP) derived from the degradation of larger polymers or synthesized for commercial purposes are ubiquitous in the environment and have found their way into food supplies and water systems where human consumption is inevitable. We hypothesize that MP ingestion promotes CVD risk and atherosclerosis. To test this idea, we supplied ApoE-/- mice with normal chow and either normal water or that containing polystyrene (PS) beads (0.5 μm in diameter; dose of 1 μg/ml). After 20 weeks of exposure, we evaluated lesion formation and gene expression data in cardiovascular tissue. We observed that mice consuming the PS water had increased lesion size compared to those mice consuming normal water. but no changes in plasma lipids or cytokines. An RNAseq analysis of collected aortae identified 1622 differentially expressed genes between the groups, consisting of 414 that were up-regulated and 1208 that were down-regulated. , , , # Differential gene expression in aortae isolated from mice consuming normal water or that containing polystyrene beads

The data are contained in two Excel files, one for up-regulated genes and one for down-regulated genes.

* PS: polystyrene beads

Each Excel file has columns consisting of:

* level in mice drinking PS water (standardized average of read count)

* level in mice drinking normal water (standardized average of read count)

* log2 fold change (ratio of gene expression between the treatment and control group expressed as logarithm of base 2)

* p-value (p-value in Wald test)

* padj-value (corrected p-value of multiple hypothesis tests)

* gene name

微塑料（Microplastics, MP）指源自大型聚合物降解或为商业用途合成的塑料微粒，在环境中广泛分布，已进入食品供应链与水体系统，人类通过饮食摄入微塑料已不可避免。本研究提出假说：摄入微塑料会升高心血管疾病（Cardiovascular Disease, CVD）风险并促进动脉粥样硬化发生。为验证该假说，我们为ApoE-/-小鼠（载脂蛋白E基因敲除小鼠）提供普通饲料，并分别饲喂正常饮水或添加直径0.5 μm聚苯乙烯（Polystyrene, PS）微球的饮水（剂量为1 μg/ml）。暴露20周后，我们对小鼠心血管组织的斑块形成情况与基因表达数据进行了评估。结果显示，与饲喂正常饮水的对照组小鼠相比，饲喂PS微球饮水的处理组小鼠动脉粥样硬化病变体积显著增大，但两组小鼠的血浆脂质与细胞因子水平未出现显著差异。对采集的主动脉组织进行RNA测序（RNA Sequencing, RNA-seq）分析后，两组共鉴定出1622个差异表达基因，其中414个基因表达上调，1208个基因表达下调。
# 饲喂正常饮水与聚苯乙烯微球饮水小鼠的主动脉组织差异基因表达分析

本数据集包含两个Excel文件，分别存储上调基因与下调基因的分析结果。
* PS：聚苯乙烯微球

每个Excel文件均包含以下列：
* PS饲喂组小鼠的基因表达水平（测序读段计数的标准化平均值）
* 正常饮水组小鼠的基因表达水平（测序读段计数的标准化平均值）
* log₂倍数变化（处理组与对照组的基因表达比值的以2为底的对数值）
* p值（Wald检验所得的p值）
* 校正p值（多重假设检验校正后的p值）
* 基因名称