Heidelberg BAG (HeidelBAG)

The HeidelBAG unites macromolecular crystallography of EMBL Heidelberg and the Heidelberg University Biochemistry Center (BZH). Central to all projects are large assemblies involved in chromosome organization and transcription control, in nuclear and cytoplasmic RNA regulation and modification, in translational control by ribosome associated factors or further in protein targeting, membrane protein insertion or post-translational modification. While O. Barabas from EMBL has left the BAG, Svetlana Dodonova and Sebastian Eustermann at EMBL will join and add projects on chromatin organization. Nearly all projects make use of integrated structural biology techniques (MX, SAXS/SANS, NMR, cryo-EM, AI approaches) that are combined with complementary methods, a strategy that warranties the continuous success of the BAG. More and more the detailed architecture and mechanistic description of entire macromolecular machines come into focus. The BAG reports on 17 publications and 30 PDB entries.

HeidelBAG整合了欧洲分子生物学实验室海德堡分部（EMBL Heidelberg）与海德堡大学生物化学中心（BZH）的大分子晶体学研究资源。所有研究项目的核心均围绕参与以下过程的大型大分子复合物展开：染色体组织与转录调控、细胞核及细胞质内的RNA调控与修饰、核糖体相关因子介导的翻译调控，以及蛋白质靶向定位、膜蛋白插入或翻译后修饰等过程。尽管来自欧洲分子生物学实验室的O. Barabas已离开HeidelBAG，但该实验室的Svetlana Dodonova与Sebastian Eustermann将加入团队，并新增染色质组织相关研究项目。几乎所有研究项目均采用整合结构生物学技术（包含MX、SAXS/SANS、NMR、冷冻电镜（cryo-EM）及人工智能方法），并结合互补性实验手段，这一策略保障了HeidelBAG的持续产出与学术成功。当前研究的焦点愈发聚焦于完整大分子机器的精细结构解析与作用机制阐释。截至目前，HeidelBAG已累计发表17篇学术论文，并向蛋白质数据库（PDB）提交了30条结构条目。