A lateral flow strip based on gold nanoparticles to detect 6-monoacetylmorphine in oral fluid

We used lateral flow strips (LFS) based on gold nanoparticles (AuNPs) to detect 6-monoacetylmorphine (6-MAM; heroin’s unique metabolite) in oral fluid samples. In this competitive lateral chromatographic immunoassay, the 6-MAM was chemically synthesized and conjugated to bovine serum albumin (BSA). The results were qualitatively detected via the color change of the test line. By using a proper sample pad, a suitable NC membrane and a customized sponge device adsorbed the oral fluid directly from mouth, and total test time was 3 minutes. The sensitivity of the assay was 4.0 ng ml-1 without any cross-reactivity with ten normal drugs, which are widely subject to abuse, including morphine and codeine. This test could be easily used on site to detect heroin in oral fluid, and it could be a promising product in the future including for driving under the influence.

本研究采用基于金纳米颗粒（Gold Nanoparticles, AuNPs）的侧向流试纸条（Lateral Flow Strips, LFS），对口腔液（Oral Fluid）样本中的单乙酰吗啡（6-Monoacetylmorphine, 6-MAM，海洛因的特异性代谢物）进行检测。在该竞争性侧向色谱免疫分析法（Competitive Lateral Chromatographic Immunoassay）中，我们将6-MAM进行化学合成并与牛血清白蛋白（Bovine Serum Albumin, BSA）偶联，检测结果通过检测线（Test Line）的颜色变化实现定性判定。通过选用适配的样品垫（Sample Pad）、硝酸纤维素膜（Nitrocellulose Membrane, NC膜），并采用定制化海绵装置（Customized Sponge Device）直接从口腔采集口腔液样本，整个检测流程总耗时仅为3分钟。该检测方法的灵敏度可达4.0纳克每毫升，且与10种常见滥用药物（包括吗啡（Morphine）与可待因（Codeine））均无交叉反应性（Cross-reactivity）。本检测手段可便捷应用于口腔液中海洛因的现场筛查，未来有望在受药物影响驾驶（Driving Under the Influence）等场景中展现应用潜力。