Ag85B-ESAT-6-specific T cell responses following neonatal and adult immunization with Ag85B-ESAT-6 in the CAF01 adjuvant.

aIFN-γ secreting cells (ISC)/106 splenocytes were determined after 48 hr of culture with antigen and IL-2 (bioassay), TNF-α, IL-17, IFN-γ and IL-5 (ELISA) after 72 hr of culture with antigen. Cytokine production by unstimulated splenocytes from mice immunized with Ag85B-ESAT-6/CAF01 and antigen stimulated/unstimulated splenocytes from mice immunized with Ag85B-ESAT-6 alone or CAF01 alone was below the limit of detection. bFollowing 6 hr culture with antigen and co-stimulation (CD28/CD49d), percent of cytokine-producing cells determined by flow cytometry. Unstimulated splenocytes from mice immunized with Ag85B-ESAT-6/CAF01 and antigen stimulated/unstimulated splenocytes from unimmunised mice were <0.2%. cNS: p>0.05. dND: not done.