Expression of miRNAs in human colorectal carcinoma cells upon autophagy induction

The expression profile of miRNAs during the response of human colon cancer cells (HT29s) to 5-FU treatment and nutrient starvation was examined using miRNA microarray analysis. Combining with the following real time PCR and bioinformatics analysis, the physiological importance of the miRNA-autophagy interconnection is far more likely to be elucidated. In the study presented here, autophagy induction was confirmed in both 5-FU-treated HT29 cells and nutrient-starved HT29 cells. These cells were used to acquire expression profiles of all human miRNAs annotated in miRBase 18.0