Additional file 1 of Identification of genomic regions affecting grain peroxidase activity in bread wheat using genome-wide association study

Additional file 1 : Table S1. The POD activity (U·min− 1·g− 1) in 207 wheat accessions of each environment and BLUP. Table S2. Analysis of variance (ANOVA) and broad-sense heritability for POD activity based on three environments. Table S3. Marker-trait associations for POD activity in the associated population analyzed by the mixed linear model (MLM). Table S4. Number of superior alleles across 20 significantly associated SNPs in the genome of 207 wheat varieties. Table S5. Primers designed based on KASP markers AX-109420494 and AX-111134186. Table S6. The relationship between superior and inferior alleles of KASP markers to POD activity. Table S7. The expression values of high-confidence POD genes within 10 Mb physical intervals from the significant SNPs. Table S8. The expression of TraesCS2D02G583000 between the varieties in association population based on the genotypes AX-94769224 and AX-110482619. Table S9. The significant SNPs of GWAS analysis conducted by the expression levels of TraesCS2D02G583000. Table S10. The false discovery rate of the 20 significant SNPs.

附加文件1：表S1：207份小麦种质资源在各环境下及最佳线性无偏估计（Best Linear Unbiased Prediction, BLUP）下的过氧化物酶（Peroxidase, POD）活性（单位：U·min⁻¹·g⁻¹）。
表S2：基于3个环境的过氧化物酶活性方差分析（Analysis of Variance, ANOVA）及广义遗传力。
表S3：通过混合线性模型（Mixed Linear Model, MLM）分析关联群体中过氧化物酶活性与分子标记的关联情况。
表S4：207份小麦品种基因组中20个显著关联单核苷酸多态性（Single Nucleotide Polymorphism, SNP）位点的优异等位基因数量。
表S5：基于竞争性等位基因特异性PCR（Kompetitive Allele Specific PCR, KASP）标记AX-109420494与AX-111134186设计的引物。
表S6：竞争性等位基因特异性PCR标记的优异与不利等位基因与过氧化物酶活性的关联关系。
表S7：距显著单核苷酸多态性位点10 Mb物理区间内的高置信度过氧化物酶基因的表达量。
表S8：基于基因型AX-94769224与AX-110482619，关联群体中各品种TraesCS2D02G583000基因的表达情况。
表S9：以TraesCS2D02G583000基因表达量为表型进行全基因组关联分析（Genome-Wide Association Study, GWAS）得到的显著单核苷酸多态性位点。
表S10：20个显著单核苷酸多态性位点的错误发现率（False Discovery Rate, FDR）。