Tissue-specific DNA methylation of the Chicken and Pig

DNA methylation of multiple tissues of the pig and chicken was investigated at a single-base resolution for functional annotation of the genomes using Reduced Representation Bisulfite Sequencing (RRBS). This study aimed at cataloging the DNA methylome of FAANG-reference tissues (liver, spleen, lung, muscle, adipose, hypothalamus, cerebral cortex and cerebellum) of two adult animals in collaboration with Dr. Huaijun Zhou, University of California, Davis, CA. The RRBS data was generated to profile tissue-specific DNA methylation patterns and to support functional annotation of the genomes for an integrative analysis such as RNA-Seq. To enhance the genome-wide CpG coverage, the RRBS libraries were constructed using a double-enzyme (MspI and TaqaI) digestion and an increase of size-selected fragments between 40 - 350bp. This study is part of the FAANG project, promoting rapid prepublication of data to support the research community. These data are released under Fort Lauderdale principles, as confirmed in the Toronto Statement (Toronto International Data Release Workshop. Birney et al. 2009, Pre-publication data sharing. Nature 461:168-170). Any use of this dataset must abide by the FAANG data sharing principles. Data producers reserve the right to make the first publication of a global analysis of this data. If you are unsure if you are allowed to publish on this dataset, please contact, [e-mail], [e-mail] and [e-mail] to enquire. The full guidelines can be found at http://www.faang.org/data-share-principle".