Replication Data for: Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections

Pseudomonas aeruginosa is a relevant pathogen in chronic respiratory infections, which are usually associated with biofilm formation, complicating in vitro modeling and effective treatment strategies. While P. aeruginosa can coexist with several microorganisms, its association with Staphylococcus aureus is widespread in cystic fibrosis (CF) patients and other bronchiectasis. Finding a reliable and straightforward in vitro model to study long-term P. aeruginosa infections is extremely hard due to the secretion of highly virulent toxins that compromise the model within less than 10 h. Several optimizations, including the use of bovine serum albumin (BSA) and extracellular matrix proteins, led to enhanced A549 cell viability up to 30 h post-infection. Within this time frame, we developed P. aeruginosa biofilms, explored host-pathogen interactions, and delved deeper into the relationship between P. aeruginosa and S. aureus. Additionally, ciprofloxacin treatment was evaluated, revealing changes and differences in antibiotic susceptibility and underlying significant differences between bacterial strains.

铜绿假单胞菌（Pseudomonas aeruginosa）是慢性呼吸道感染的重要致病菌，此类感染常伴随生物被膜（biofilm）形成，这一特性为体外建模与有效治疗策略的开发带来了显著复杂性。尽管铜绿假单胞菌可与多种微生物共存，但其与金黄色葡萄球菌（Staphylococcus aureus）的共存在囊性纤维化（cystic fibrosis, CF）患者及其他支气管扩张症群体中极为普遍。由于该菌会分泌高毒力毒素，可在感染后10小时内破坏体外模型，因此构建可靠且简便的体外模型以研究铜绿假单胞菌的长期感染过程一直极具难度。此前已有多项优化手段，包括使用牛血清白蛋白（bovine serum albumin, BSA）与细胞外基质蛋白，可将A549细胞的存活率维持至感染后30小时。在此时间范围内，我们成功构建了铜绿假单胞菌的生物被膜，探究了宿主-病原体互作（host-pathogen interactions）机制，并进一步深入分析了铜绿假单胞菌与金黄色葡萄球菌之间的相互关系。此外，本研究还评估了环丙沙星（ciprofloxacin）的治疗效果，结果显示不同菌株的抗生素敏感性存在变化与差异，且菌株间的内在差异亦十分显著。