Hemizygous loci of Mytilus galloprovincialis genome.

We employed the allelic structural variation detection pipeline to determine the hemizygous regions of the primary assembly. Tandem repeats in genome assembly were identify using trf v4.09. PacBio HiFi reads were aligned to the primary assembly using minimap2 v2.17-r94. Structural variations in the primary assembly were identified using pbsv v2.9.0 (https://github.com/PacificBiosciences/pbsv). The primary assembly exhibited widespread hemizygous regions, with 8.79% deletions of the total genome length (Fig. 4). When insertions were taken into account, the rate of hemizygosity of the primary assembly increased to 16.32%.