Data Sources .docx

Low-grade glioma (LGG) is a prevalent and lethal primary brain malignancy, with most patients succumbing to recurrence and progression. The signal transducer and activator of transcription (STAT) family has long been implicated in tumor initiation and progression. However, a comprehensive evaluation of the expression status and overall function of STAT genes in LGG remains largely unreported. In this study, we investigated the association between the expression of STAT family genes and the progression of LGG. Through a comprehensive analysis that combined bioinformatics screening and validation assays, we determined that STAT1, STAT3, and STAT5A were upregulated and contributed to the malignant progression of LGG. Notably, our findings suggest that STAT3 is a critical prognostic marker that regulates the progression of LGG. STAT3 emerged as the most significant prognostic indicator governing the advancement of LGG. Additionally, our inquiry into the STAT3-binding proteins and differentially expressed-correlated genes (DEGs) revealed that STAT3 played a pivotal role in the progression of LGG by stimulating the expression of STAT1, FOXO1, and MYC. In summary, our recent study conducted a thorough analysis of the STAT family genes and revealed that directing therapeutic interventions towards STAT3 holds potential as a viable strategy for treating patients with LGG.

低级别胶质瘤（Low-grade glioma, LGG）是一种常见且致命的原发性脑部恶性肿瘤，多数患者最终会因肿瘤复发与病情进展而死亡。长期以来，信号转导与转录激活因子（signal transducer and activator of transcription, STAT）家族已被证实与肿瘤的发生及进展密切相关。然而，目前针对LGG中STAT家族基因的表达状态与整体功能的系统性评估仍鲜有报道。
本研究探讨了STAT家族基因表达与LGG进展之间的关联。通过整合生物信息学筛选与验证实验的多维度分析，我们证实STAT1、STAT3及STAT5A呈上调表达，并参与了LGG的恶性进展。值得注意的是，本研究结果显示STAT3是调控LGG进展的关键预后标志物，堪称影响LGG进展的最具影响力的预后指标。
此外，我们对STAT3结合蛋白及差异表达相关基因（differentially expressed-correlated genes, DEGs）的探究显示，STAT3可通过上调STAT1、FOXO1与MYC的表达，在LGG进展中发挥关键调控作用。
综上，本研究对STAT家族基因进行了全面分析，结果提示靶向STAT3的治疗策略有望成为LGG患者的潜在治疗方案。