Data for "Tailoring the Surface Pore Morphology of Bioceramic Scaffolds through Colloidal Processing for Bone Tissue Engineering", PLOS ONE, 2024.

In this study, porous bioceramic scaffolds are developed with two materials, β-tricalcium phosphate (β-TCP) and hydroxyapatite (HA), with order of 10 micron-scale surface pores and approximately 40-60% volume porosity fabricated by soft templating of oil. Suitable oil and surfactant concentrations are determined for the creation of particle-stabilized emulsions with nearly spherical pores, as well as the capillary suspensions with elongated pores. The bioceramic scaffolds are then assessed for their ability to support osteoblast adhesion and growth, for applications as scaffolds for bone regeneration. The porous scaffolds’ surfaces are compared to denser surfaces of the same material, where only submicron porosity arise from partial sintering, to interrogate the impact of surface topography on cell behavior. On the denser surfaces where no large pores are templated, β-TCP supports a larger number of osteoblasts compared to HA. Templated porosity significantly impacts the morphology and growth of the osteoblasts. Amongst the pore morphologies, the capillary suspension demonstrates enhanced biological function, whereas the emulsion performs the poorest. The β-TCP capillary suspension scaffold appears to provide the most favorable conditions for the osteoblasts.

本研究采用β-磷酸三钙（β-tricalcium phosphate, β-TCP）与羟基磷灰石（hydroxyapatite, HA）两种材料，通过软模板油法制备得到兼具10微米级表面孔隙、体积孔隙率约40%~60%的多孔生物陶瓷支架（porous bioceramic scaffold）。研究确定了适用于制备具有近乎球形孔隙的颗粒稳定乳液（particle-stabilized emulsion）以及具有细长孔隙的毛细管悬浮液（capillary suspension）的油相与表面活性剂浓度。随后评估了该生物陶瓷支架支持成骨细胞（osteoblast）黏附与增殖的能力，以应用于骨再生支架（bone regeneration scaffold）领域。为探究表面形貌（surface topography）对细胞行为的影响，本研究将多孔支架的表面与同种材料仅通过部分烧结（sintering）形成亚微米孔隙（submicron porosity）的致密表面进行对比。在未通过模板制备大孔隙的致密表面上，β-TCP相较于HA可支持更多的成骨细胞。模板法制备的孔隙结构对成骨细胞的形态与增殖具有显著影响。在不同孔隙形貌的支架中，毛细管悬浮液法制备的支架展现出更优的生物学性能，而乳液法制备的支架性能最差。β-TCP毛细管悬浮液支架似乎为成骨细胞提供了最适宜的生长微环境。