Wastewater treatment using microbial enzymatic mixture

Abstract The effectiveness of the use of microbial enzymes as a wastewater treatment method depends on factors such as temperature, pressure, and oxygenation level, among others. However, very few scientific studies have provided real data regarding the evaluation of the applicability of this type of treatment. This research was therefore carried out to evaluate the effect of temperature and aerobic treatment time on the level of treatment of wastewater pollutants, with a mixture composed of enzymes and aerobic microorganisms under environmental conditions in the city of Huancayo, Peru. For this purpose, synthetic wastewater samples were prepared, considering a value of CODinitial= 800 mg L-1. One group of experiments was conducted at a 20°C controlled temperature and another group without control. The following average kinetic constants were obtained as results (Ko= 1.662 d-1, Kc=2.53 mg COD mg-1 VSS) when the T°= 20°C. For the experiments where the T° was not controlled, the average constants were: Ko= 1.217 d-1, Kc=1.24 mg COD mg-1 SSV. We observed from the measurements that the pollutant removal was adjusted to the kinetic model proposed by Orozco, and the biomass production was adjusted to the stoichiometric equation of biomass production with endogenous respiration. Therefore, we concluded that the effect of temperature control and treatment time on the level of purification caused by the microbial enzymatic product is significant at a 95% confidence level.

摘要 以微生物酶类作为污水处理手段的效能，受温度、压力、供氧水平等多种因素影响。然而，目前鲜有科学研究提供此类处理技术适用性评估的实测数据。本研究旨在评估温度与好氧处理时长对污水污染物去除效果的影响，实验采用酶与好氧微生物的混合体系，在秘鲁万卡约市的现场环境条件下开展。
为此，我们制备了初始化学需氧量（COD）为800 mg·L⁻¹的合成污水样品。实验分为两组：一组在20℃控温条件下进行，另一组未进行温度控制。当温度维持20℃时，实验得到如下平均动力学常数：Ko=1.662 d⁻¹，Kc=2.53 mg COD·mg⁻¹ VSS；对于未控温实验组，平均动力学常数分别为：Ko=1.217 d⁻¹，Kc=1.24 mg COD·mg⁻¹ SSV。
经检测分析可知，污染物去除过程符合Orozco提出的动力学模型，而生物质生成过程则符合伴随内源呼吸的生物质生成化学计量方程。综上，在95%置信水平下，温度控制与处理时长对该微生物酶制剂的净化效果具有显著影响。