NanoString CosMx data from human xenografts treated with engineered T cells

Engineered T cells demonstrate varied and limited efficacy in solid tumors, so genetic modulation of these therapies improves their potency. We engineered T cell receptor (TCR) and chimeric antigen receptor (CAR) T cells to consitutively express tumor necrosis alpha (TNF-a) to maintain secretion upon antigen engagement. We applied spatially resolved single cell transcriptomics (on the NanoString CosMx Spatial Molecular Imaging platform) to human xenografts treated with TCR- and CAR-T therapies with and without TNF-a-armored engineering. FFPE blocks (N=23) were sectioned on to 12 slides (1-3 samples per slide) for processing on the NanoString CosMx Spatial Molecular Imager platform per the manufacturer protocol, using the (1.0) Human RNA Universal Cell Characterization, with the (1.0) Human RNA Universal Cell Characterization Add-On Probe Kit and the (1.0) Human RNA Universal Cell Segmentation and (1.0) Human RNA IO PanCK/CD45 Morphology Kit. Data was processed on the AtoMx Spatial Informatics Platform (v2.0) and exported as raw CSV files (including the count matrix, cell metadata, transcripts, polygons, FOV positions) for analysis.