The expression of LRRN4 was correlated with the progression and prognosis of colon adenocarcinoma (COAD) patients

Abstract Our present study aims to investigate the value of LRRN4 in the progression and prognosis of COAD patients. All COAD and adjacent sample data was downloaded from TCGA database. Survival analysis was performed according to Kaplan-Meier method. The real-time quantitative PCR and immunohistochemistry analysis were conducted for validation in cell lines and tissues. The GSEA was conducted to find functional KEGG pathways. Multivariate Cox regression proportional hazard mode was used to determine whether LRRN4 expression was an independent prognostic factor. The LRRN4 expression in COAD samples were significantly higher than that in adjacent samples, which was consistent with our experiments in cell lines and tissues. Along with the increase of TNM Stage, LRRN4 expression had an increasing tendency. The COAD patients with high LRRN4 expression showed undesirable prognoses. Additionally, the TGF-β signaling pathway, WNT signaling pathway and other 25 pathways were significantly activated in the high LRRN4 expression group. In conclusion, high LRRN4 expression was closely related to the onset of COAD and it was a poor prognostic factor for COAD patients.

摘要
本研究旨在探讨亮氨酸重复序列神经元蛋白4（LRRN4）在结肠腺癌（COAD）患者疾病进展与预后中的价值。本研究所有结肠腺癌及癌旁组织样本数据均下载自癌症基因组图谱（TCGA）数据库。采用卡普兰-迈耶法（Kaplan-Meier）进行生存分析。通过实时定量聚合酶链反应（real-time quantitative PCR）及免疫组织化学分析，对细胞系与组织样本进行验证。采用基因集富集分析（GSEA）筛选功能相关的KEGG通路。采用多变量Cox比例风险回归模型，明确LRRN4表达是否为独立预后因子。结肠腺癌组织中LRRN4的表达水平显著高于癌旁组织，这一结果与我们在细胞系及组织样本中的实验结果一致。LRRN4的表达水平随TNM分期升高呈递增趋势。LRRN4高表达的结肠腺癌患者预后较差。此外，在LRRN4高表达组中，转化生长因子-β（TGF-β）信号通路、WNT信号通路及其余25条通路均显著激活。综上，LRRN4高表达与结肠腺癌的发生密切相关，且可作为结肠腺癌患者的不良预后因子。