Levels of mtDNA content in peripheral blood WBC pre- and post-CCI TBI at 25 hours.

Mean ± SEM CT quantifications from real-time PCR assays of COI-1, COI-1A, ND4-1 amplicons from peripheral whole blood. Relative mtDNA copy number was determined, for each gene, by the ratio of mtDNA gene copy number to an internal control of nuclear DNA (nDNA), to a 16S rRNA qRT-PCR. The RQ ratio is proportional to mtDNA copy number in each cell. Nicotinamide adenine dinuclueotide dehydrogenase (NADH) subunit 4mitochondrial (ND4-1). Cytochrome c oxidase subunit (COI-1) and I-A (COI-1A).Levels of mtDNA content in peripheral blood WBC pre- and post-CCI TBI at 25 hours.