Mapping the Drosophila developmental and aging atlas by single cell mRNA-seq

Single-cell mRNA sequencing (scRNA-seq) technologies are reshaping current cell-type classification system. In previous studies, we constructed the Mouse Cell Atlas (MCA) and Human Cell Landscape (HCL) to catalog all cell types by collecting scRNA-seq data. Howerver, the systematic study for organism-level dynamic changes of cellular states across fruit fly (Drosophila melanogaster) life span are still lacking. Here, we constructed the Drosophila cell Landscape covering different development periods using Microwell-seq protocol. The Drosophila cell landscape provides a valuable resource for studying cross-sepciess development, maturation and aging. Overall design: About 162,495 cells derived from Drosophila whole-body single-cell dissociation and profiling at 21 day (D21), D26, D31, D36, and D41 after laying eggs (nearly 25-30 fruit flies per timepoint).