Early onset of adult deafness in the Rhodesian Ridgeback is associated with in-frame deletion in the EPS8L2 gene

In order to recruit EOAD-affected and normal hearing dogs, we conducted an online survey in 2020 to private dog owners of Rhodesian Ridgebacks who agreed to participate in scientific research. DNA was extracted from buccal swab samples collected by dog owners and extracted by Illumina, Inc. Genotypes of the dogs were collected by using custom Illumina Canine high-density SNP arrays.

为招募罹患早发性阿尔茨海默病（Early-Onset Alzheimer's Disease，EOAD）及听力正常的罗得西亚脊背犬（Rhodesian Ridgebacks），我们于2020年面向饲养该品种犬且同意参与科学研究的私人饲主开展了一项线上调查。样本由犬只饲主自行采集口腔拭子，随后由Illumina公司完成DNA提取工作。研究团队通过定制化Illumina犬类高密度单核苷酸多态性（Single Nucleotide Polymorphism，SNP）芯片完成了受试犬的基因分型检测。