GckPVT neuronal networks

Transcriptomic characterization of the glucokinase-expressing neurons of the paraventricular thalamus in the mouse, using Transcribing Ribosome Affinity Purification (TRAP) sequencing. Overall design: Transgenic mice expressing cre recombinase in the glucokinase-expressing cells were injected in the aPVT with an AAV-DIO-L10-GFP encoding a L10 ribosomal protein-GFP fusion protein that integrates in ribosomes. Ten days later, after an overnight fast, aPVT was dissected, lysed and the ribosomes were immunoprecipitated with an anti-GFP antibody. RNA-seq was then performed on the immunoprecipitated ribosomal fraction (output) and on the non-immunoprecipitated (input) material. 4 biological repricates were used.