SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in November 2016

Flow cytometry data was collected in November 2016, in waters off South Australia.

The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.

Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

流式细胞术（Flow Cytometry）数据采集于2016年11月，采集区域为澳大利亚南部近岸海域。本研究的核心目标为建立澳大利亚南部大陆架海域生态系统的基础背景资料，阐明上升流/咸水溢流事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作作为澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目的组成部分，在RV Ngerin号科考船的航次中开展。各航次均针对叶绿素荧光最大值（Deep Chlorophyll Maximum, DCM）层的物理、化学及生物学特性开展调查，并采集了超微型浮游植物、细菌与病毒的流式细胞术数据。本研究共布设6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）以及SAM5CB/B5（35.008°S，135.198°E）；剩余1个站位为位于袋鼠岛西南侧的外海站位B1（36.188°S，136.178°E）。需注意的是，结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）以及船舶平均航速（约9节），可判定各站位所采集的水团均不相同。研究期间还偶尔从袋鼠岛国家参考站位（National Reference Station, NRS，35.832°S，136.447°E）以及发生咸水溢流的南澳大利亚斯宾塞湾口锚泊站（SAM8SG，35.25°S，136.690°E）采集了补充样本。