Modulation of the atherosclerosis-related miRNA and target genes profile of endothelial cells by extra-virgin olive oil and sunflower oil. Raw sequence reads

BackgroundEndothelial cells are very involved in the development of atherosclerosis. They are very exposed to the effects of triglycerides-rich lipoprotein (TRLs). However, it is not known the effect that these TRLs can have on the miRNAs expression of endothelial cells according the type of diet.AimsWe wanted to analyze the differences between the effect of TRLs obtained after a high fat meal with two type of oil (extra virgin olive oil (EVOO) and sunflower oil (SO)) on the miRNA profile involved in pathways related to atherosclerosis in HUVECs.ResultsWe found 28 upregulated differentially expressed (DE) miRNAs with EVOO-derived TRLs which could regulate 22 exclusive potential target genes related to atherosclerosis signaling. We also found 21 downregulated DE miRNAs with EVOO-derived TRLs (or upregulated with SO-derived TRLs) which could regulate 20 exclusive potential target genes related to atherosclerosis signaling. These miRNAs were mainly involved in the regulation of angiogenesis, with a predominance of an anti-angiogenic effect with EVOO-derived TRLs. Other miRNAs upregulated with SO-derived TRLs were involved in different cardiovascular diseases. Pathways for the exclusive potential target genes obtained from the upregulated DE miRNA with EVOO-derived TRLs were mainly involved in lipid metabolism and inflammatory and defense response, while those with SO-derived TRLs were mainly involved in lipid metabolic process.ConclusionsEVOO-derived TRLs seem to produce a most favorable atheroprotective profile than SO-derived TRLs. This study provides alternative mechanisms and insights on the protective role of EVOO against atherogenic process through the regulation of miRNAs in endothelial cells.