DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 3.

DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 3. The stretch of gp 63 previously described as encoding the functional domain of the molecule was amplified by PCR, cloned into pcr 2.1 vector and bidirectionally sequenced. Sequences from a panel of 41 parasite isolates were aligned and 45 alleles of the fragment could be identified based on indel and SNPs presented among cloned domains. Haplotypes of polymorphisms (indel and SNPs) that could be detected in at least two clones of the gp63 domain in a single isolate, and in at least two parasite isolates in the collection were considered alleles. Corte de Pedra is a region highly endemic for American tegumentary leishmaniasis (ATL) due to L. braziliensis. Several forms of ATL occur and close to 1,500 new cases are diagnosed in the region each year.<br><br>

巴西东北部Corte de Pedra地区巴西利什曼原虫（Leishmania braziliensis）自然分离株中编码利什曼蛋白酶（leishmanolysin）功能结构域的gp63片段DNA序列：等位基因3。此前被报道可编码该分子功能结构域的gp63区段通过聚合酶链式反应（PCR）扩增，克隆至pCR2.1载体并完成双向测序。对41株寄生虫分离株的序列进行比对后，基于各克隆结构域间存在的插入缺失（indel）与单核苷酸多态性（SNPs），可鉴定出该片段的45种等位基因。将满足以下条件的多态性（插入缺失与单核苷酸多态性）单倍型（haplotypes）认定为等位基因：该单倍型可在单个分离株的至少两个gp63结构域克隆中被检出，且在本次收集的至少两株寄生虫分离株中存在。Corte de Pedra地区是巴西利什曼原虫引发的美洲皮肤利什曼病（American tegumentary leishmaniasis, ATL）高流行区域，该地区存在多种美洲皮肤利什曼病临床表现类型，每年新确诊病例近1500例。