FRET data for manuscript "Light-Induced Triplet–Triplet Electron Resonance Spectroscopy with Orthogonal Chromophores allows Pulsed ESR Distance Measurements between FRET Labels"

Three FRET datasets for each of the samples/experiments measured in manuscript "Light-Induced Triplet-Triplet Electron Resonance Spectroscopy with Orthogonal Chromophores allows Pulsed ESR Distance Measurements between FRET Labels". Each dataset contains, in separate tabs: the UV/Vis absorbance spectrum of the acceptor+peptide molecule and calculated epsilon (spectrum normalised to molar extinction coefficient of acceptor), the raw and normalised fluorescence emission spectra of the donor+peptide molecule, the calculated overlap, and the raw fluorescence emission spectrum of the acceptor+peptide+donor molecule. A tab labelled values lists the values taken from literature to carry out calculations alongside values taken/calculated from the collected data. Experimental and computational methods are described in the SUPPORTING INFORMATION of the manuscript. DOI of the manuscript to be added after acceptance. A README is included to accompany datasets. Contents: EBdonorTPPacceptor.xlsx - dataset corresponding to FRET measurements of sample labelled <strong>[2] </strong>in manuscript. TPPacceptorZnTPPdonor(567ex).xlsx - dataset corresponding to FRET measurements of sample labelled <strong>[1] </strong>in manuscript, where 567 nm laser excitation was used: TPP was taken to be acceptor and ZnTPP was taken to be donor. TPPdonorZnTPPacceptor(510ex).xlsx - dataset corresponding to FRET measurements of sample labelled <strong>[1] </strong>in manuscript, where 510 nm laser excitation was used: TPP was taken to be donor and ZnTPP was taken to be acceptor.

本数据集包含针对论文《Light-Induced Triplet-Triplet Electron Resonance Spectroscopy with Orthogonal Chromophores allows Pulsed ESR Distance Measurements between FRET Labels》中所检测的全部样本/实验对应的3组荧光共振能量转移（Fluorescence Resonance Energy Transfer, FRET）数据集。每组数据集均以独立工作表（tab）的形式存储以下内容：受体-肽分子的紫外-可见吸收光谱，以及经受体摩尔消光系数归一化后的计算摩尔消光系数（ε）；供体-肽分子的原始与归一化荧光发射光谱；计算得到的光谱重叠积分；以及受体-肽-供体三元复合物的原始荧光发射光谱。其中名为`values`的工作表收录了从文献中获取的计算所需参数，以及从采集数据中提取或计算得到的相关数值。实验与计算方法详见该论文的支持信息（SUPPORTING INFORMATION）。该论文的数字对象标识符（Digital Object Identifier, DOI）将在论文录用后补充。本数据集附带README说明文件。各数据集文件详情如下：1. EBdonorTPPacceptor.xlsx：对应论文中标注为<strong>[2]</strong>的样本的FRET测量数据集。2. TPPacceptorZnTPPdonor(567ex).xlsx：对应论文中标注为<strong>[1]</strong>的样本的FRET测量数据集，该实验采用567 nm激光激发：以四苯基卟啉（Tetraphenylporphyrin, TPP）为受体，锌四苯基卟啉（Zinc Tetraphenylporphyrin, ZnTPP）为供体。3. TPPdonorZnTPPacceptor(510ex).xlsx：对应论文中标注为<strong>[1]</strong>的样本的FRET测量数据集，该实验采用510 nm激光激发：以TPP为供体，ZnTPP为受体。