Exploring differentially expressed genes in hypothalamic (HT), pars tuberalis (PT) and pineal gland (PG) transcriptomes in different sexual behaviour phenotypes in rams using RNA-seq

To understand the mechanisms of sexual behaviour in rams, transcriptomic sequencing technology was used to identify differentially expressed genes in key brain tissues We then performed gene expression profiling analysis using data obtained from RNA-seq of hypothalamus (HT), pars tuberalis (PT) and pineal gland (PG) in rams with different sexual behaviours Overall design: The PT, HT and PG transcriptomes of 12 Rasa Aragonesa rams (6 sexually active (A) and 6 not active (NA) for each tissue) were generated by Novaseq 6000 platform Identifcation of differentially expressed genes (DEGs) in the 3 studied tissues. The multifactorial design was used where the state (A or NA) was defined as the primary contrast condition and the tissue (PT, HT, and PG) as the secondary contrast condition