MOESM1 of Suppressed N fixation and diazotrophs after four decades of fertilization

Additional file 1: Table S1. Physicochemical soil properties in bulk soil and rhizosphere soil among different fertilization treatments. Table S2. The relative abundance of dominant diazotrophic genera among different treatments. Table S3. Pairwise t-tests for the relative abundance of dominant diazotrophic genera between bulk soil and rhizosphere soil in different fertilization treatments. Table S4. Comparison of diazotrophic alpha-diversity indexes among different fertilization treatments. Table S5. Pairwise t-testing of diazotrophic alpha-diversity in bulk soil and rhizosphere soil. Table S6. Pairwise t-tests of nitrogen fixation rates in bulk soil and rhizosphere soil in different fertilization treatments. Table S7. ADONIS double factor analysis for the diazotrophic community. Table S8. Pairwise t-tests of relative abundance of main ecological clusters within the diazotrophic community in bulk soil and rhizosphere soil under different fertilization treatments. Table S9. The network properties for the main ecological clusters of the diazotrophic community. Table S10. Operational taxonomic unit (OTU) count properties of important species for nitrogen fixation rates found by the Random Forest model in the three main ecological clusters. Table S11. The correlations (r) and significance (p) were determined using a Mantel test between the diazotrophic community and environmental variables in bulk soil and rhizosphere soil. Table S12. Spearman correlation between physicochemical soil properties and diazotrophic alpha-diversity. Table S13. Spearman correlation between physicochemical soil properties and relative abundance of the main diazotrophic ecological clusters.

附加文件1：表S1 不同施肥处理下非根际土壤（bulk soil）与根际土壤（rhizosphere soil）的土壤理化性质。表S2 不同处理下优势固氮菌属的相对丰度。表S3 不同施肥处理下，非根际土壤与根际土壤中优势固氮菌属相对丰度的两两t检验。表S4 不同施肥处理下固氮微生物α多样性指数对比。表S5 非根际土壤与根际土壤中固氮微生物α多样性的两两t检验。表S6 不同施肥处理下，非根际土壤与根际土壤固氮速率的两两t检验。表S7 固氮微生物群落的ADONIS双因素分析。表S8 不同施肥处理下，非根际土壤与根际土壤中固氮微生物群落内主要生态类群相对丰度的两两t检验。表S9 固氮微生物群落主要生态类群的网络属性。表S10 随机森林模型筛选出的与固氮速率相关的重要物种，在3个主要生态类群中的操作分类单元（Operational Taxonomic Unit，OTU）计数特征。表S11 非根际土壤与根际土壤中，固氮微生物群落与环境变量间的相关性系数（r）与显著性（p）由Mantel检验确定。表S12 土壤理化性质与固氮微生物α多样性的斯皮尔曼相关性。表S13 土壤理化性质与固氮微生物主要生态类群相对丰度的斯皮尔曼相关性。