DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 19.

DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 19. The stretch of gp 63 previously described as encoding the functional domain of the molecule was amplified by PCR, cloned into pcr 2.1 vector and bidirectionally sequenced. Sequences from a panel of 41 parasite isolates were aligned and 45 alleles of the fragment could be identified based on indel and SNPs presented among cloned domains. Haplotypes of polymorphisms (indel and SNPs) that could be detected in at least two clones of the gp63 domain in a single isolate, and in at least two parasite isolates in the collection were considered alleles. Corte de Pedra is a region highly endemic for American tegumentary leishmaniasis (ATL) due to L. braziliensis. Several forms of ATL occur and close to 1,500 new cases are diagnosed in the region each year.

来自巴西东北部科尔特德佩德拉地区巴西利什曼原虫（Leishmania braziliensis）自然分离株中编码利什曼溶素（leishmanolysin）功能域的gp63片段DNA序列：等位基因19。此前被文献报道可编码该分子功能域的gp63区段通过聚合酶链式反应（PCR）扩增，被克隆至PCR2.1载体并进行双向测序。对41株寄生虫分离株的序列进行多序列比对后，基于克隆功能域间存在的插入缺失（indel）与单核苷酸多态性（SNPs），可鉴定出该片段的45种等位基因。若某一多态性（插入缺失与单核苷酸多态性）单倍型可在单份分离株的至少两个gp63功能域克隆中被检测到，且可在本次收集的至少两份寄生虫分离株中检出，则将其定义为等位基因。科尔特德佩德拉是由巴西利什曼原虫引发的美洲皮肤利什曼病（ATL）高流行地区。该地区存在多种临床类型的美洲皮肤利什曼病，每年新确诊病例接近1500例。