Molecular plasticity under climate change: a quantitative proteomics approach to better understand the response of two Iberian freshwater fish to heat and pH stress

We conducted a proteome-level study on the endangered Mira chub, Squalius torgalensis, which inhabits unstable habitats, enduring harsh summers with high temperatures and frequent droughts. We assessed the effect of warming and acidification, independently and combined on protein expression and phosphorylation in gills and muscle using tandem mass tags labelling proteomics.