Transcriptomic profiling of MAFB-KD THP-1 macrophages infected with Mycobacterium tuberculosis

PMA-stimulated THP-1 cells were treated with siRNA against MAFB gene. MAFB-KD macrophages were infected with Mycobacterium tuberculosis at an MOI of 1. Infected macrophages were also stimulated with IFN-g at 10 ng/mL. RNA was purified from MAFB-KD macrophages infected with M. tuberculosis, followed by mRNA purification with using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Libraries were constructed with NEBNext Ultra II Directional RNA Library Prep Kit (NEB). Libraries were examined with Qubit fluorometer (Thermo Fisher Scientific) and GenNext NGS Library Quantification Kit (TOYBO) for quantity control, and DNA High Sensitivity Kit on Agilent Bioanalyzer 2100 (Agilent) for quality control, respectively. Paired-end sequencing (75 bp x 2) of libraries were performed using NextSeq500 (Illumina).