Changes in mouse kidney glomerular gene expression following dendrin knockout

Glomerular podocyte cells are critical for the function of the renal ultrafiltration barrier. The highly specialized cell-cell junction of podocytes, the slit diaphragm, has a central role in the filtration barrier. Dendrin is a poorly characterized cytosolic component of the slit diaphragm in where it interacts with nephrin and Cd2ap. In this study, we have generated a dendrin knockout mouse line and explored the molecular interactions of dendrin. Dendrin-deficient mice were viable, fertile and had normal life span. To reveal the glomerular gene expression changes in the dendrin knockout mouse, Affymetrix Mouse Genome 430 2.0 Array were used to profiling the dendrin knockout and control glomeruli. Three dendrin knockout and three littermate control mice at age of 13 months were used to profile glomerular transcriptomes. Total glomerular RNA was extracted and hyrbridized on the Mouse Genome 430 2.0 Array according to standard procedures.肾小球足细胞（glomerular podocyte cells）对肾脏超滤屏障的功能发挥至关重要的作用。足细胞高度特化的细胞间连接结构——裂孔隔膜（slit diaphragm），在滤过屏障中居于核心地位。树突蛋白（Dendrin）是裂孔隔膜中一种功能尚未明确的胞质组分，可与肾病蛋白（nephrin）及CD2相关蛋白（Cd2ap）发生相互作用。本研究构建了树突蛋白敲除小鼠品系，并对树突蛋白的分子相互作用展开探究。树突蛋白缺陷小鼠可正常存活、繁殖，且寿命无异常。为揭示树突蛋白敲除小鼠的肾小球基因表达变化，本研究采用Affymetrix小鼠基因组430 2.0芯片（Affymetrix Mouse Genome 430 2.0 Array）对树突蛋白敲除小鼠与对照小鼠的肾小球进行转录组表达谱分析。本研究选用13月龄的3只树突蛋白敲除小鼠及3只同窝对照小鼠开展肾小球转录组分析。按照标准实验流程，提取全部肾小球总RNA，并将其与小鼠基因组430 2.0芯片进行杂交。