Expression profile of the nucleoredoxin-like 2 gene in the mouse brain using a beta-galactosidase knock-in reporter strain

The nucleoredoxin-like 2 (Nxnl2) gene reporter stain (Nxnl2R) was created using Velocigene stem cells. Those cells carry a beta-galactosidase reporter knock-in in the Nxnl2 locus replacing the coding sequence of the gene for the two proteins, rod-derived cone viability factor (RdCVF2) and the thioredoxin like protein RdCVF2L. Brain sections of the Nxnl2R/+ and its negative control (Nxnl2+/+), both of C57Bl/6N genetic background were stained using beta-galactosidase enzymatic reaction. The staining was read using Allen Mouse Brain Atlas version 2 (2011). Here we present brain section images of a mouse model designed to expression profile of the Nxnl2 gene.

本数据集涉及的核氧化还原蛋白样2（nucleoredoxin-like 2, Nxnl2）基因报告染色模型（Nxnl2R），通过Velocigene干细胞系构建获得。该干细胞系在Nxnl2基因座处整合了β-半乳糖苷酶（beta-galactosidase）报告基因敲入（knock-in）元件，替换了该基因编码两种蛋白的序列：杆细胞衍生视锥细胞存活因子（rod-derived cone viability factor, RdCVF2）与硫氧还蛋白样蛋白RdCVF2L（thioredoxin like protein RdCVF2L）。本实验选取均为C57BL/6N遗传背景的Nxnl2R/+杂合小鼠及其阴性对照Nxnl2+/+纯合小鼠的脑组织切片，采用β-半乳糖苷酶酶促反应进行染色。染色结果通过艾伦小鼠脑图谱第二版（2011，Allen Mouse Brain Atlas version 2 (2011)）完成判读。本数据集公开了用于解析Nxnl2基因表达谱的小鼠模型脑切片图像。