Optimization of ultrasound-assisted extraction of sheep abomasum protein concentrates by response surface methodology and evaluation of their properties

Abstract The aim of this study was to extract sheep abomasum protein concentrates (SAPC) by ultrasound-assisted extraction (UAE) and to investigate the properties of SAPC. Response surface methodology and Box-Behnken design were applied to determine the optimal parameters for UAE. The maximum water-soluble protein concentration was 320.5 mg protein/g dry raw material under the optimal conditions with ultrasonic treatment time of 28 min, ultrasonic power of 450 W, liquid/solid ratio of 25 mL/g, and pH of 10. Compare with conventional extraction method (CEM), the UAE not only provided the higher protein concentration and yield but also required much shorter extraction times. Additionally, the SAPC obtained by UAE demonstrated better solubility, emulsifying properties, foaming properties and oil holding capacity as compared to CEM. However, use of the UAE method did not significantly increase the water holding capacity of SAPC.

摘要 本研究旨在通过超声辅助提取法（ultrasound-assisted extraction, UAE）提取绵羊皱胃蛋白浓缩物（sheep abomasum protein concentrates, SAPC），并对其相关特性展开研究。本研究采用响应面法与Box-Behnken设计，确定了超声辅助提取的最优工艺参数。在最优条件下，即超声处理时间28 min、超声功率450 W、液料比25 mL/g、pH值10，水溶性蛋白浓度可达320.5 mg蛋白/g干原料。与传统提取法（conventional extraction method, CEM）相比，超声辅助提取法不仅可获得更高的蛋白浓度与提取率，同时所需提取时间更短。此外，相较于传统提取法所得产物，超声辅助提取法制备的SAPC展现出更优的溶解性、乳化特性、起泡特性与持油能力，但该方法并未显著提升SAPC的持水能力。