CIL:20093

Camera lucida sketches of the zebrafish embryo at selected stages. This image is from the Pharyngula period at the 35 hour Prim-22 stage. The animal pole is to the top for the early stages, and anterior is to the top later, except for the two animal polar (AP) views shown below their side view counterparts for germ-ring and shield gastrulas. Face views are shown during cleavage and blastula stages. After shield stage, the views are of the embryo's left side, but before the shield arises one cannot reliably ascertain which side is which. Pigmentation is omitted. Arrowheads indicate the early appearance of some key diagnostic features at the following stages: 1 k-cell: YSL nuclei. Dome: the doming yolk syncytium. Germ ring: germ ring. Shield: embryonic shield. 75%-epiboly: Brachet's cleft. 90%-epiboly: blastoderm margin closing over the yolk plug. Bud: polster. 3-somite: third somite. 6-somite: eye primordium (upper arrow), Kupffer's vesicle (lower). 10-somite: otic placode. 21-somite: lens primordium. Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). Prim-16: heart. High-pec: pectoral fin bud. Composite image CIL: 20098 is a low resolution image with a scale bar = 250 microns that shows the entire set of image. Images correspond to Fig 1 of Developmental Dynamics 203:253-310 (1995). This material is reproduced with permission of John Wiley & Sons, Inc. Copyright 1995 WILEY-LISS, INC.

投影描绘法绘制的斑马鱼胚胎特定发育阶段素描图。本图取自咽胚期（Pharyngula period）35小时的Prim-22阶段。胚胎早期阶段以动物极（animal pole）朝上为视角标准，后期则调整为前端（anterior）朝上；仅胚环期与原盾期的原肠胚两张动物极（animal polar, AP）视图置于其对应侧视图下方，为例外情况。卵裂期（cleavage）与囊胚期（blastula）阶段采用正面视图展示。原盾期之后的所有视图均为胚胎左侧视角，但在原盾结构形成前，无法可靠区分胚胎左右侧。本图未展示胚胎色素沉着情况。箭头标注了以下各发育阶段部分关键诊断特征的早期出现位置：
1000细胞期：卵黄合胞体层（yolk syncytial layer, YSL）细胞核；
隆起期：隆起的卵黄合胞体；
胚环期：胚环；
原盾期：胚胎原盾；
75%外包期：布拉切特裂隙（Brachet's cleft）；
90%外包期：胚层边缘向卵黄栓闭合；
芽基期：胚端隆起；
3体节期：第三体节；
6体节期：眼原基（上方箭头）、库普弗囊泡（Kupffer's vesicle，下方箭头）；
10体节期：耳基板；
21体节期：晶状体原基；
Prim-6阶段：后侧线原基（背侧）、孵化腺（卵黄球表面）；
Prim-16阶段：心脏；
高鳍期：胸鳍芽。
组合图像CIL:20098为低分辨率图像，配有250微米比例尺，完整展示了本数据集的全部图像。本图像对应《Developmental Dynamics》203卷第253-310页（1995年）的图1。本文档经John Wiley & Sons, Inc.授权转载，版权归1995年 WILEY-LISS, INC.所有。