Additional file 2: of Identification of transcription factor genes involved in anthocyanin biosynthesis in carrot (Daucus carota L.) using RNA-Seq

Sequence and primer properties used to analyses 11 candidate genes and GAPDH (Additional file 2.1), PCR efficiencies calculated for primer pairs using 4 fold (based on mean Ct values observed for triplicates) dilution series of cDNA mix from all 30 samples (Pfaffl 2001; Additional file 2.2), relative expression ratio of 11 candidate genes in purple vs non-purple calli/tissue (Average of biological samples) calculated with following formula (Pfaffl 2001; Additional file 2.3); trimmed mean of M-values (TMM) of 11 candidate genes in purple and non-purple calli/tissue (Additional file 2.4). (XLSX 58 kb)

用于分析11个候选基因及甘油醛-3-磷酸脱氢酶（GAPDH）的序列与引物特性（附加文件2.1）；基于30份样本混合互补脱氧核糖核酸（cDNA）的4倍梯度稀释系列（以三次重复的平均循环阈值（Ct）值为依据），针对各引物对计算得到的聚合酶链式反应（PCR）扩增效率（Pfaffl 2001；附加文件2.2）；11个候选基因在紫色愈伤组织与非紫色愈伤组织/组织中的相对表达比值（生物学重复样本的平均值），采用下述公式计算得到（Pfaffl 2001；附加文件2.3）；11个候选基因在紫色与非紫色愈伤组织/组织中的M值截尾均值（trimmed mean of M-values, TMM）（附加文件2.4）。（XLSX格式，文件大小58 kb）