mRNA microarray analysis in colorectal cancer tissues

Colorectal cancer (CRC) is one of the most common malignant tumors of digestive tract, and its incidence rate and mortality rate are all on the rise. Despite the substantial development in early diagnosis and therapeutic approaches, CRC continues to have high incidence and mortality rates. Most advanced patients have a poor prognosis because of advanced recurrence and metastasis.One of the main reasons is that the mechanisms underlying CRC remain unclear. To further investage the role of circRNA in CRC, a mRNA micoarray was performed. Fresh tumor and matched normal tissues were collected from CRC patients in the First Affiliated Hospital of NanjingMedical University after surgery . None of the patients received chemotherapy nor radiotherapy preoperatively before surgery. All samples were frozen and stored at -80◦C conservation.