Metadata record for the manuscript: Synergistic activation of mutant TERT promoter by Sp1 and GABPA in BRAFV600E-driven human cancers

<b>Summary</b>

This
metadata record provides details of the data supporting the claims of the
related manuscript: “Synergistic activation of mutant TERT
promoter by Sp1 and GABPA in BRAFV600E-driven human cancers”.

The
data consist of 31 Excel .xlsx format spreadsheets and a Word .docx document.

The
related study tested the hypothesis that activated extracellular
signal-regulated kinase (ERK) may be recruited to mutant telomerase reverse
transcriptase (TERT) promoter by pioneer transcription factors.

<b>Data
access</b>

The
31 Excel .xlsx spreadsheets underlie the figures in the related manuscript, and
are named according to the figure and subfigure they underlie (e.g., Figure 1B
1.xlsx). These are openly available and are included together with this metadata record, and they contain data concerning qRT-PCR, dual-luciferase reporter assay, ChIP and TRAP assays described in the related manuscript. The sequence homology analsysis (underlying
Supplementary Figure 8) is openly available and is also included together with this
metadata record. Data for the Co-IP assay supporting Figure 3 are in the file WB-CoIPs.tiff and are available upon request to the corresponding author, Prof. Peng Hou: [e-mail].

<b>数据集概述</b>

本元数据记录详细说明了支撑相关学术论文《BRAFV600E驱动的人类癌症中Sp1与GABPA对突变型TERT启动子的协同激活》核心主张的相关数据。
本次公开的数据包含31份Excel .xlsx格式电子表格与1份Word .docx格式文档。
本项研究验证了如下假说：激活的细胞外信号调节激酶（extracellular signal-regulated kinase, ERK）可通过先驱转录因子被招募至突变型端粒酶逆转录酶（telomerase reverse transcriptase, TERT）启动子区域。

<b>数据获取说明</b>

支撑该论文各图表的31份Excel .xlsx电子表格均按照其所对应的图表及子图表命名（例如图1B 1.xlsx），现已随本元数据记录公开提供，其中包含论文中提及的实时定量逆转录聚合酶链反应（quantitative real-time polymerase chain reaction, qRT-PCR）、双荧光素酶报告基因检测（dual-luciferase reporter assay）、染色质免疫沉淀（Chromatin Immunoprecipitation, ChIP）及端粒重复序列扩增（telomeric repeat amplification protocol, TRAP）检测相关实验数据。支撑补充图8的序列同源性分析数据也已随本元数据记录一并公开。
支撑图3的免疫共沉淀（Co-immunoprecipitation, Co-IP）实验数据存储于文件WB-CoIPs.tiff中，相关数据需向通讯作者侯鹏教授（[e-mail]）申请获取。