Unveiling the Secrets of Exercise: Decoding Exosomal Molecular Patterns in Humans During Exercise Using Metabolomic and Lipidomic Screening and Advanced Computational Analysis.

The main objective of this project was to conduct a detailed analysis of EVs isolated from cerebrospinal fluid (CSF) and serum, as well as whole CSF, and plasma obtained from young, healthy individuals before and after a 90-minute run at 75-80% of their maximal oxygen consumption (VO₂max). Regular physical activity is essential for overall health, regulation of the whole body energy metabolism, and proper brain function. During exercise, skeletal muscles and adipose tissue communicate with other vital organs, including brain, through the spectrum of heterogenous molecules, including myokines and adipokines, metabolites, lipids, and microRNAs. This complex molecular crosstalk helps the body maintain allostasis and possibly contributes to the exercise-enhanced neurogenesis and synaptic plasticity. The exact molecular patterns that mediate this communication are still largely unknown. This study aims to investigate lipidomic profiles of healthy adults before and after acute intensive endurance excercise to gain insight into the mechanisms of adaptive response to excercise.

本项目的核心研究目标为：对年轻健康个体在以75%~80%最大摄氧量（VO₂max）完成90分钟跑步运动前后采集的样本开展详细分析，这些样本包括从脑脊液（cerebrospinal fluid, CSF）和血清中分离得到的细胞外囊泡（Extracellular Vesicles, EVs）、完整脑脊液以及血浆。规律体育运动对机体整体健康、全身能量代谢调控以及正常脑功能维持均不可或缺。运动过程中，骨骼肌与脂肪组织可通过一系列异质性分子——包括肌因子、脂肪因子、代谢物、脂质以及微小RNA——与包括大脑在内的各类重要器官进行信号互作。这种复杂的分子串扰可帮助机体维持动态内稳态，且可能参与运动诱导的神经发生与突触可塑性过程。目前介导该信号交流的确切分子模式仍尚不明确。本研究旨在探究健康成年人急性高强度耐力运动前后的脂质组学特征，以深入解析运动适应性应答的潜在分子机制。