Transcriptomic analysis of Wild-type and Mfsd7c-/-knockout brain embryos

Mfsd7c is expressed in CNS blood vessels. Deletion of Mfsd7c results in embryonic lethality with signs of hypoxia. We performed transcriptomic analysis of genes in brains of E14.5 wild-type (WT) and Mfsd7c-knockout (KO) embryos. The total mRNA from whole brain of E14.5 WT and KO embryos was isolated for RNA sequencing using Novaseq6000 (the newest IIIumina platform).Mfsd7c在中枢神经系统（Central Nervous System, CNS）的血管中表达。敲除Mfsd7c会引发胚胎致死，并伴随缺氧征象。我们对E14.5天的野生型（Wild-type, WT）与Mfsd7c敲除（Mfsd7c-knockout, KO）胚胎的脑组织开展了基因转录组分析。我们提取了E14.5天野生型与敲除胚胎全脑的总mRNA，使用Novaseq6000（当前最新的Illumina测序平台）进行RNA测序。