Gene expression signatures for Intrahepatic cholangiocarcinoma. Gene expression signatures for Intrahepatic cholangiocarcinoma

CircRNA microarray was used to screen significantly up-regulated circRNAs in paired ICC and non-tumor tissues 7 paired ICC and non-tumor tissues was used to screen significantly CircRNAs Overall design: 7 paired ICC and non-tumor tissues，We have completed the Arraystar Human circRNA Array v2 analysis of the 14 samples that you submitted. Total RNA from each sample was quantified using the NanoDrop ND-1000. The sample preparation and microarray hybridization were performed based on the Arraystar’s standard protocols. Briefly, total RNAs were digested with Rnase R (Epicentre, Inc.) to remove linear RNAs and enrich circular RNAs. Then, the enriched circular RNAs were amplified and transcribed into fluorescent cRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar). The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array v2 (8x15K, Arraystar). After having washed the slides, the arrays were scanned by the Agilent Scanner G2505C.