Landscape of open chromatin regions in Rice upon exposure to abiotic stresses

The goal of the study is to illustrate how various abiotic stresses reshape the chromatin architecture of the Rice genome to fine tune gene expression. Overall design: Oryza sativa L. Japonica nipponbare was grown in a plant growth chamber (Conviron®) at 28°C under a photoperiod of 16 h/8 h. 14-day old seedlings were subjected to heat (42°C for 30 min and 3 h), cold (4°C for 12 h and 24 h), and salt (250 mM NaCl for 2 h and 6 h) stress. The 14-day old seedlings grown at 28°C were taken as the control sample. Open chromatin was extracted using Formaldehyde Assisted Isolation of Regulatory Elements (FAIRE-seq) from two biological replicates. RNA-seq was performed on two biological replicates