File S1 - HER2 Targeted Molecular MR Imaging Using a De Novo Designed Protein Contrast Agent

(A) Magnetic resonance images and image intensities of the mouse tumor pre-blocked by affibody ZHER2:342 (B) Purification of PEGylated ProCA1-affi-m (C) Far-UV CD and Tryptophan fluorescent spectra (D) Optical spectrum of ProCA1-affi-m conjugated with NIR dye Cy5.5 (E) NIR images of cultured cancer cells (AU565 and SKOV-3) with high expression of HER2. The scale bar value is 25 µm. (F) Immunofluorescent histology of tumor tissue (Xenograft SKOV-3 model) stained by HER2 antibody and ProCA1-affi-m. The scale bar value is 100 µm. (G) Blood circulation of GdCl3, ProCA1-affi and ProCA1-affi-m in Xenograft nude mice (H) Toxicity analysis by clinical chemistry assay. (DOC)