five

Individual serological data (ELISA, WB).

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NIAID Data Ecosystem2026-03-10 收录
下载链接:
https://figshare.com/articles/dataset/Low_antibody_prevalence_against_i_Bacillus_cereus_i_biovar_i_anthracis_i_in_Ta_National_Park_C_te_d_Ivoire_indicates_high_rate_of_lethal_infections_in_wildlife/5427577
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Table containing information on the origin of individual samples and results for ELISA and Western Blot testing. Given are the mean measured ELISA OD450nm values for all samples tested (sorted by species) and their respective values for the assay internal negative control. ELISA measurements were conducted in a 1:500 dilution and the mean of the blanks was subtracted. To assess the quality of the measurements, the coefficient of variation was calculated and readings were rerun if it was higher than 0.2. The human positive control for primate testing was titrated in an 8 step log2 serial dilution on the plate with a starting concentration of 1:4000 and yielded a titre of 512000. For duikers, each plate was validated through inclusion of a pooled positive control serum from goats vaccinated with the B. anthracis Sterne spore live vaccine. This was titrated in an 8 step log2 serial dilution on the plate with a starting concentration of 1:1000 and yielded a titre of 128000. LF and PA Western Blot were performed with 1:1000 dilutions for all samples and controls. (XLSX)
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2017-10-03
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