INFLUENCE OF PRETREATMENTS ON CRYSTALLINITY AND ENZYMATIC HYDROLYSIS IN SUGAR CANE RESIDUES

ABSTRACT This research evaluated the effect of different delignification pretreatments (enzymatic and organosolv), on the crystallinity and enzymatic hydrolysis of harvested sugar cane residues. The Crystallinity Index (CrI), the Relative Number of Intensity (Ir), the degree of cellulose mercerization (IIC-%), and the Global Index of Saccharification (GIS) were used as measurement parameters for six different substrates obtained from sugar cane residues (tops and leaves) by different processes. In this characterization, the spectroscopic ty Techniques of Fourier Transform infrared spectroscopy (FTIR), X-ray diffraction and scanning eElectron microscopy (SEM) were used. Substrates to which only organosolv pretreatment was applied, without any further treatment, presented good behavior for the enzymatic hydrolysis and a high CrI, possibly due to the increase of the crystallinity by elimination of amorphous material.

摘要 本研究评估了不同脱木质素预处理方式（酶法与有机溶剂法（organosolv））对收获后甘蔗残渣结晶度与酶解效率的影响。本研究以通过不同工艺从甘蔗残渣（梢部与叶片）中制备得到的六种底物为研究对象，采用结晶度指数（CrI）、强度相对值（Ir）、纤维素丝光化程度（IIC-%）及总糖化指数（GIS）作为检测参数。本次表征采用傅里叶变换红外光谱（FTIR）、X射线衍射与扫描电子显微镜（SEM）技术。仅施加有机溶剂预处理且未进行后续处理的底物，其酶解性能优异且结晶度指数较高，这可能源于无定形物质的去除提升了底物的结晶度。