The production of FST from lung adenocarcinoma cells.

(A) The tumor cells were incubated in 2.5% and 10% FCS-IMEM culture medium, respectively. The non-tumor lung tissue cells were incubated in 10% FCS-IMEM culture medium. The supernatants of cultured cells were harvested at the indicated time point and FST levels were detected by ELISA. ○ Lung adenocarcinoma cells with 10% FCS-IMDM medium. Δ Lung adenocarcinoma cells with 2.5% FCS-IMDM medium. • Non-tumor lung tissue cells with 10% FCS-IMEM medium. (B) FST protein expression in human lung adenocarcinoma cell line A549 cells was examined by immunocytochemical staining with anti-FST antibody (Anti-FST). A procedural background control (Control) was performed using normal mouse IgG instead of anti-FST antibody.