Raw reads and assembled sequence of Inverse PCR products

This is the supporting data for the inverse PCR test for the formation of circular intermediates. The .ab1 and .fasta files can be accessed using the BioEdit.exe software .To further explore the recombination regions of the "segment 1", "segment 2" and "segment 3", please align the assembled sequences against pEFM9-1 (accessed by NCBI Accession Number: CP098744.1) using the online BLASTn strategy. Similarly, please align the assembled sequence against pEFT30-1(accessed by NCBI Accession Number: CP113829.1) to explore the recombination region of the "acquired segment".

本数据集为环状中间体形成的反向PCR检测实验提供配套支撑数据。可通过BioEdit.exe软件读取其中的.ab1格式测序峰图文件与.fasta格式序列文件。若需进一步探究"片段1""片段2"与"片段3"的重组区域，请采用在线基础局部比对搜索工具（BLASTn）策略，将组装得到的多条序列与参考质粒pEFM9-1（NCBI（美国国家生物技术信息中心，National Center for Biotechnology Information）登录号：CP098744.1）进行序列比对。同理，请将该组装序列与pEFT30-1（NCBI登录号：CP113829.1）进行序列比对，以探究"获得片段"的重组区域。