Anticancer and antioxidant Holuthuria edulis
收藏doi.org2023-11-29 更新2025-03-26 收录
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http://doi.org/10.17632/jfkzp7vvwt.1
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资源简介：
This research describes the antioxidant and anticancer activity of sea cucumber extract Holothuria edulis from Labuan Bajo. Sea cucumbers are extracted using three methods, namely maceration, soxhletation and ultrasonication. The data displayed is related to the calculation of toxicity data for the BSLT method, antioxidants and anticancer MCF-7 cells.
The BSLT method toxicity calculation is as follows, the calculation of toxicity (IC50) was carried out using probit analysis.
Number of dead larvae
% death = ------------------------ x 100%
Number of test larvae
The IC50 was then calculated
A blank – A sample
% inhibition = -------------------- x 100%
A blank
The IC50 was then calculated:
The absorbance of treated cells – absorbance of control media
% cell death = -------------------------------------------------------------------- x 100%
The absorbance of control cell – Absorbance of control media
本研究详细阐述了来自Labuan Bajo地区的海参提取物Holothuria edulis的抗氧化和抗癌活性。海参的提取采用三种方法，即浸泡、索氏提取和超声处理。所展示的数据与BSLT方法的毒性数据计算、抗氧化剂和抗癌MCF-7细胞的活性相关。BSLT方法毒性计算如下，毒性（IC50）的计算通过概率分析完成。
死亡幼虫数量
% 死亡率 =
------------------------
测试幼虫数量
随后计算IC50值
% 抑制率 =
-------------------------
空白对照
空白对照
随后计算IC50值：
处理细胞的光吸收值 – 对照介质的吸收值
% 细胞死亡率 =
--------------------------------------------------------------------
对照细胞的吸收值 – 对照介质的吸收值
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