Porcisia hertigi: Functional Annotations

We annotated the assemblies using the MAKER2 annotation pipeline (version 2.31.10) over two rounds: 1) an evidence-based annotation round using EST, mRNA-seq and protein homology evidence from TriTrypDB (release-47) along with our repeat-masking output. The second round is an ab initio round using AUGUSTUS, with the pre-trained Leishmania tarentolae as the model organism. After completion of all annotation rounds, we assigned functional annotations from the Uniprot and Pfam databases using blast+ and InterProScan.Description

我们采用MAKER2注释流程（版本2.31.10）对基因组组装序列开展两轮注释：第一轮为基于证据的注释阶段，使用取自TriTrypDB数据库（第47版）的表达序列标签（Expressed Sequence Tag, EST）、mRNA测序（mRNA-seq）数据与蛋白质同源性证据，并结合本研究的重复序列屏蔽输出结果；第二轮为从头预测注释阶段，采用AUGUSTUS软件，以预先训练完成的塔氏利什曼虫（Leishmania tarentolae）作为模式生物。完成全部注释流程后，我们借助BLAST+工具与InterProScan软件，从UniProt与Pfam数据库中匹配得到功能注释信息。