Genome-wide translational profiling of amygdala Crh-expressing

Here we translationally profiled the transcriptome of Crh-expressing neurons (Crh neurons) within the CeA following fear conditioning (FC) and fear extinction (EXT) in mice using translating ribosome affinity purification (TRAP) followed by RNA sequencing. A tone alone group (TA) was used as a control group. Individual animals were used as each sample (3 groups: FC, EXT and TA; n=10 males and females per group).本研究采用翻译核糖体亲和纯化（translating ribosome affinity purification, TRAP）结合RNA测序技术，对小鼠中央杏仁核（central amygdala, CeA）内表达Crh的神经元（Crh神经元）在经历条件性恐惧（fear conditioning, FC）与恐惧消退（fear extinction, EXT）处理后的转录组进行翻译谱分析。本研究设置纯音调组（tone alone group, TA）作为对照。每个样本对应一只独立个体，实验共分为3组：FC组、EXT组与TA组，每组样本量为10，涵盖雄性与雌性个体。