Identification of gene modulating vascular inflammation using chromosome 2 fragment substitutions and RNA sequencing [miRNA-Seq]

Chromosome 2 introgression from normotensive Brown Norway (BN) rats into hypertensive Dahl salt-sensitive (SS) background (consomic S2B) reduced blood pressure (BP) and vascular inflammation under normal salt diet (NSD). We hypothesized that BN chromosome 2 contains anti-inflammatory genes that could reduce BP elevation and vascular inflammation in rats fed NSD and high salt diet (HSD). We used chromosome 2 fragment substitutions to map chromosome 2 portion associated with vascular inflammation changes and next generation sequencing (NGS) to profile microRNAs in thoracic descending aorta of SS and congenic rats fed NSD or HSD.将正常血压布朗挪威（Brown Norway, BN）大鼠的2号染色体渐渗至高血压达尔盐敏感（Dahl salt-sensitive, SS）大鼠的遗传背景中，构建得到同源替换系S2B（consomic S2B）；该品系可在正常盐饮食（normal salt diet, NSD）喂养条件下降低大鼠血压（blood pressure, BP）与血管炎症。本研究假设BN大鼠2号染色体携带有抗炎基因，能够在正常盐饮食与高盐饮食（high salt diet, HSD）喂养的大鼠中抑制血压升高并减轻血管炎症。我们采用2号染色体片段替换技术定位与血管炎症变化相关的2号染色体区域，并通过下一代测序（next generation sequencing, NGS）分析正常盐饮食或高盐饮食喂养的SS大鼠及同类系大鼠（congenic rats）胸降主动脉中的微小RNA（microRNAs）表达谱。