Illumina MiSeq sequenced 16S library of Microbial Community Response to Oil and Corexit in Coastal Water Mesocosms

Two independent mesocosm experiments were carried out in August (Mesocosm#1) and October of 2015 (Mesocosm#2) using coastal water collected near Galveston Bay, Texas. Four mesocosm treatments were prepared in triplicate for this study: (1) Control (seawater only); (2) WAF (oil + seawater); (3) Chemically-enhanced WAF (CE-WAF; oil + Corexit + seawater); (4) Diluted CE-WAF (1:10 dilution). Initial mesocosm volumes were 79L. Prior to experiment initiation, phytoplankton were collected from coastal Galveston, TX waters and added to each tank to stimulate interactions between phytoplankton, oil, and Corexit. Starting at time-point zero and every 12 hours thereafter, water was collected from each mesocosm in clean, opaque Nalgene bottles with PTFE-lined diaphragm pumps. DNA was extracted from filtered water samples and sequenced using barcoded primers specific for the V4 hypervariable region of the 16S rRNA gene.

本研究于2015年8月（中宇宙实验#1）及2015年10月（中宇宙实验#2）开展两组独立的中型受控生态系统（mesocosm）实验，实验用水采自美国得克萨斯州加尔维斯顿湾附近的近岸海域。本研究设置4种中宇宙处理组，每组均设置3个生物学重复：(1) 对照组（仅含海水）；(2) 水容油相组（WAF，油+海水）；(3) 化学强化型水容油相组（CE-WAF，油+柯西特（Corexit）+海水）；(4) 稀释型化学强化水容油相组（按1:10比例稀释）。每组中宇宙系统的初始体积为79升。实验启动前，研究人员从得克萨斯州加尔维斯顿近岸海域采集浮游植物，并接种至每个实验容器中，以促进浮游植物、石油与柯西特之间的相互作用。自计时零点起，之后每隔12小时，研究人员使用内衬聚四氟乙烯（PTFE）的隔膜泵，将每个中宇宙系统中的水样采集至洁净的不透明Nalgene瓶中。研究人员对过滤后的水样提取DNA，并使用针对16S rRNA基因V4高变区的条形码引物进行测序。