Transcriptomic catalog isoform classification.

The Plasmodium falciparum 3D7 transcriptomic catalog constructed from long-read sequencing data is shown for 5,568 reference transcripts (S2A) and 12,495 novel isoforms (S2B). Details are shown for the classification.txt output from SQANTI3 (columns 1−32, headers in orange; for explanation of column headers, see S1 & S2 Tables in Tardaguila et al [35]). The tama_merge_trans.txt output from TAMA merge [27] is shown in columns 33−38 (headers in blue). Column 33 (sources) refers to candidate non-redundant isoforms assigned by TAMA collapse [27] for each long-read RNA-Seq dataset for alignment described in S1 Table. Columns 34 (start_wobble_list) and 35 (end_wobble_list) refer to the "wobble", or range of exon start and end coordinates, respectively among candidate isoforms from different data sources merged into the final isoform. Columns 36 (exon_start_support) and 37 (exon_end_support) list the source data information of candidate isoforms used for assigning the 5′ and 3′ ends, respectively of the final merged isoform. Column 38 (all_source_trans) lists all candidate isoforms and data sources used for merging. (XLSX)