Generation of HAdv-7-neutralizing monoclonal antibodies.

aThe ascites titer against rAdMHE3 is expressed as the reciprocal of the ascites dilution and was determined by indirect ELISA.bEach ascites (1 ml) was purified by octanoic acid–ammonium sulfate precipitation to a final volume of 150 µl and the IgG concentration was determined spectrophotometrically using a conversion factor of 1.4 mg/ml per absorbance unit at 260 nm [22].cNeutralization titer is expressed as the reciprocal of the ascites dilution and was determined as the highest dilution of ascites that protected HEp-2 cell monolayers from a visually observable CPE.d3D7 was one of the 22 MAbs isolated from mice vaccinated with rAdMHE3 virions. We used 3D7 here as a control.